Determination of Nitrogen Fixing capacity of Bacteria Isolated from the Rhizosphere soil of Crotolaria pallida from the Valley Districts of Manipur, India

In the current study an attempt was made for separation of bacteria having nitrogen fixing potential from the rhizosphere of wild leguminous plant Crotolaria pallida. Single spot each from the four valley districts of Manipur viz. Imphal east, Imphal west, Bishnupur and Thoubal was selected for the collection of soil sample. A total of six (6) bacterial isolates were isolated using Burk’s nitrogen free medium from the rhizosphere of the plant. Preliminary physiological tests and biochemical tests were being carried out for the isolates for assessment of their nature. Also, “Nitrogenase activities” of the isolates were also determined by performing the “Acetylene Reduction Assay”. Five bacterial isolates out of the six showed some nitrogenase activity even though the amounts shown were little.